salmon sperm dna (Thermo Fisher)
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Salmon Sperm Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/salmon+sperm+dna/Deoxyribonucleic+acid+sodium+salt%2C+salmon+testes/pm42243101-259-45-48
Average 95 stars, based on 1 article reviews
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Staining:Article Title: Co-enrichment of proteins in extracellular vesicles. Article Snippet: .. For on-slide PS staining, FITC-conjugated Annexin V (Biolegend, USA) was diluted 1: 50 in Annexin V binding buffer (Biolegend, USA) and incubated for 1 h. For antibody immunostaining, oligoconjugated antibodies were incubated at 5 μg/mL for 2 h diluted in PBS with 3% BSA, sheared Binding Assay:Article Title: Co-enrichment of proteins in extracellular vesicles. Article Snippet: .. For on-slide PS staining, FITC-conjugated Annexin V (Biolegend, USA) was diluted 1: 50 in Annexin V binding buffer (Biolegend, USA) and incubated for 1 h. For antibody immunostaining, oligoconjugated antibodies were incubated at 5 μg/mL for 2 h diluted in PBS with 3% BSA, sheared Incubation:Article Title: Co-enrichment of proteins in extracellular vesicles. Article Snippet: .. For on-slide PS staining, FITC-conjugated Annexin V (Biolegend, USA) was diluted 1: 50 in Annexin V binding buffer (Biolegend, USA) and incubated for 1 h. For antibody immunostaining, oligoconjugated antibodies were incubated at 5 μg/mL for 2 h diluted in PBS with 3% BSA, sheared Article Title: An integrated protocol for multiplexed DNA FISH and protein detection in large tissue sections Article Snippet: .. After fixation and permeabilization, tissue samples were incubated at 37°C inside a humidity chamber for 1 h in 70ul pre-hybridization buffer containing 50% formamide / 2x SSC / 5x Denhardt’s solution (ThermoFisher) / 50 mM sodium phosphate buffer / 1 mM EDTA (ThermoFisher) / 100 μg/mL Article Title: Large RNA polymerase II condensates are promoter-centric assemblies associated with early stages of transcription at all expressed genes Article Snippet: The following day, labeled DNA was purified using DNA Clean & Concentrator (Zymo) and labeling efficiency was assessed by UV–Vis spectrophotometry (NanoDrop). .. For probe preparation, 200 ng labeled DNA was ethanol-precipitated together with Cot-1 DNA (4 μg; Thermo), Immunostaining:Article Title: Co-enrichment of proteins in extracellular vesicles. Article Snippet: .. For on-slide PS staining, FITC-conjugated Annexin V (Biolegend, USA) was diluted 1: 50 in Annexin V binding buffer (Biolegend, USA) and incubated for 1 h. For antibody immunostaining, oligoconjugated antibodies were incubated at 5 μg/mL for 2 h diluted in PBS with 3% BSA, sheared Blocking Assay:Article Title: Single-Cell and Spatial Methods for Multimodal Functional Glycan Profiling in Tissues Article Snippet: The antibody mixture was then filtered through a EDTA-free S2 Buffer pre-wetted 50kDa filter (Sigma Millipore, UFC5050BK) at 12,500 rpm for 8 min at 4°C. .. The slides were then blocked with Blocking Buffer that contains BBDG (5% normal donkey serum, 0.05% NaN 3 in 1× TBS-T wash buffer (Sigma, 935B-09)) supplemented with 50 μg/ml mouse IgG (diluted from 1 mg/ml stock (Sigma, I5381-10mg) in S2), 50 μg/ml rat IgG (diluted from 1 mg/ml stock (Sigma, I4141-10mg) in S2), 500 μg/ml sheared other:Article Title: An integrated protocol for multiplexed DNA FISH and protein detection in large tissue sections Article Snippet: Next, to prepare the samples for hybridization, both cells and tissue samples were rinsed once then incubated overnight in a buffer containing 50% Lysis:Article Title: Reprogramming mRNA localization by targeted RNA-protein interference Article Snippet: For purification of dCas13-B2-EGFP-3xFLAG/sgRNA complex, initially, dCas13-B2-EGFP-3xFLAG was immunoprecipitated with anti-FLAG magnetic agarose beads (ThermoFisher; A36798), as described earlier, from cell lysates of HEK 293LT that were transiently expressing the protein. .. The bound RNA-λN-GST protein complex was washed with lysis buffer (50mM Tris-Cl pH 7.5, 0.5% Triton X-100, 100mM NaCl, 2.5mM MgCl 2 ) and blocked with lysis buffer containing E. coli tRNA, BSA (Sigma; 10711454001), Labeling:Article Title: Large RNA polymerase II condensates are promoter-centric assemblies associated with early stages of transcription at all expressed genes Article Snippet: The following day, labeled DNA was purified using DNA Clean & Concentrator (Zymo) and labeling efficiency was assessed by UV–Vis spectrophotometry (NanoDrop). .. For probe preparation, 200 ng labeled DNA was ethanol-precipitated together with Cot-1 DNA (4 μg; Thermo), Centrifugation:Article Title: Large RNA polymerase II condensates are promoter-centric assemblies associated with early stages of transcription at all expressed genes Article Snippet: The following day, labeled DNA was purified using DNA Clean & Concentrator (Zymo) and labeling efficiency was assessed by UV–Vis spectrophotometry (NanoDrop). .. For probe preparation, 200 ng labeled DNA was ethanol-precipitated together with Cot-1 DNA (4 μg; Thermo), |
