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salmon sperm dna  (Thermo Fisher)


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    Structured Review

    Thermo Fisher salmon sperm dna
    Salmon Sperm Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/salmon+sperm+dna/Deoxyribonucleic+acid+sodium+salt%2C+salmon+testes/pm42243101-259-45-48
    Average 95 stars, based on 1 article reviews
    salmon sperm dna - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Co-enrichment of proteins in extracellular vesicles.
    Article Snippet: .. For on-slide PS staining, FITC-conjugated Annexin V (Biolegend, USA) was diluted 1: 50 in Annexin V binding buffer (Biolegend, USA) and incubated for 1 h. For antibody immunostaining, oligoconjugated antibodies were incubated at 5 μg/mL for 2 h diluted in PBS with 3% BSA, sheared salmon sperm DNA (Thermo Fisher, USA) and 0.5% dextran sulfate (Sigma, USA), and additionally with 0.05% Saponin for cytosolic targets. ..

    Binding Assay:

    Article Title: Co-enrichment of proteins in extracellular vesicles.
    Article Snippet: .. For on-slide PS staining, FITC-conjugated Annexin V (Biolegend, USA) was diluted 1: 50 in Annexin V binding buffer (Biolegend, USA) and incubated for 1 h. For antibody immunostaining, oligoconjugated antibodies were incubated at 5 μg/mL for 2 h diluted in PBS with 3% BSA, sheared salmon sperm DNA (Thermo Fisher, USA) and 0.5% dextran sulfate (Sigma, USA), and additionally with 0.05% Saponin for cytosolic targets. ..

    Incubation:

    Article Title: Co-enrichment of proteins in extracellular vesicles.
    Article Snippet: .. For on-slide PS staining, FITC-conjugated Annexin V (Biolegend, USA) was diluted 1: 50 in Annexin V binding buffer (Biolegend, USA) and incubated for 1 h. For antibody immunostaining, oligoconjugated antibodies were incubated at 5 μg/mL for 2 h diluted in PBS with 3% BSA, sheared salmon sperm DNA (Thermo Fisher, USA) and 0.5% dextran sulfate (Sigma, USA), and additionally with 0.05% Saponin for cytosolic targets. ..

    Article Title: An integrated protocol for multiplexed DNA FISH and protein detection in large tissue sections
    Article Snippet: .. After fixation and permeabilization, tissue samples were incubated at 37°C inside a humidity chamber for 1 h in 70ul pre-hybridization buffer containing 50% formamide / 2x SSC / 5x Denhardt’s solution (ThermoFisher) / 50 mM sodium phosphate buffer / 1 mM EDTA (ThermoFisher) / 100 μg/mL salmon sperm DNA (Invitrogen) (pH 7.75 ± 0.25). .. Then the prehybridization buffer was replaced with by the hybridization mix obtained by mixing the primary FISH probes diluted at 0.6 nM per oligonucleotide, at a ratio of 1:9 vol./vol. in a buffer consisting of 55% formamide / 2.2x SSC / 5.5x Denhardt’s solution / 55 mM sodium phosphate buffer / 1.1 mM EDTA / 111 ng/μL salmon sperm DNA / 11% w/w dextran sulfate (Thermo Fisher) (pH 7.75 ± 0.25).

    Article Title: Large RNA polymerase II condensates are promoter-centric assemblies associated with early stages of transcription at all expressed genes
    Article Snippet: The following day, labeled DNA was purified using DNA Clean & Concentrator (Zymo) and labeling efficiency was assessed by UV–Vis spectrophotometry (NanoDrop). .. For probe preparation, 200 ng labeled DNA was ethanol-precipitated together with Cot-1 DNA (4 μg; Thermo), salmon sperm DNA (10 μg; Thermo), and glycogen carrier, incubated at −80°C for 1 h, pelleted by centrifugation, washed twice with 70% ethanol, and air-dried protected from light. ..

    Immunostaining:

    Article Title: Co-enrichment of proteins in extracellular vesicles.
    Article Snippet: .. For on-slide PS staining, FITC-conjugated Annexin V (Biolegend, USA) was diluted 1: 50 in Annexin V binding buffer (Biolegend, USA) and incubated for 1 h. For antibody immunostaining, oligoconjugated antibodies were incubated at 5 μg/mL for 2 h diluted in PBS with 3% BSA, sheared salmon sperm DNA (Thermo Fisher, USA) and 0.5% dextran sulfate (Sigma, USA), and additionally with 0.05% Saponin for cytosolic targets. ..

    Blocking Assay:

    Article Title: Single-Cell and Spatial Methods for Multimodal Functional Glycan Profiling in Tissues
    Article Snippet: The antibody mixture was then filtered through a EDTA-free S2 Buffer pre-wetted 50kDa filter (Sigma Millipore, UFC5050BK) at 12,500 rpm for 8 min at 4°C. .. The slides were then blocked with Blocking Buffer that contains BBDG (5% normal donkey serum, 0.05% NaN 3 in 1× TBS-T wash buffer (Sigma, 935B-09)) supplemented with 50 μg/ml mouse IgG (diluted from 1 mg/ml stock (Sigma, I5381-10mg) in S2), 50 μg/ml rat IgG (diluted from 1 mg/ml stock (Sigma, I4141-10mg) in S2), 500 μg/ml sheared salmon sperm DNA (ThermoFisher, AM9680), 50 nM oligo block (diluted from stock with 500 nM of each oligo in 1× TE pH 8.0 (Invitrogen, AM9849) and 1X Carbo-free Blocking Solution (diluted from 10X (Vector Laboratories, SP-5040-125)). .. Blocking was performed in a humidity chamber on ice during which was photobleached using Happy Lights (Verilux, VT22) for 1 h, with temperature kept below 40°C.

    other:

    Article Title: An integrated protocol for multiplexed DNA FISH and protein detection in large tissue sections
    Article Snippet: Next, to prepare the samples for hybridization, both cells and tissue samples were rinsed once then incubated overnight in a buffer containing 50% formamide (ThermoFisher) / 2x SSC / 50 mM sodium phosphate buffer, at room temperature in the dark.

    Lysis:

    Article Title: Reprogramming mRNA localization by targeted RNA-protein interference
    Article Snippet: For purification of dCas13-B2-EGFP-3xFLAG/sgRNA complex, initially, dCas13-B2-EGFP-3xFLAG was immunoprecipitated with anti-FLAG magnetic agarose beads (ThermoFisher; A36798), as described earlier, from cell lysates of HEK 293LT that were transiently expressing the protein. .. The bound RNA-λN-GST protein complex was washed with lysis buffer (50mM Tris-Cl pH 7.5, 0.5% Triton X-100, 100mM NaCl, 2.5mM MgCl 2 ) and blocked with lysis buffer containing E. coli tRNA, BSA (Sigma; 10711454001), salmon sperm DNA (Invitrogen; 15632011) and glycogen (Invitrogen; AM9510) for 30–60 min at 4°C. .. Subsequently, beads were washed 5 times with lysis buffer to remove unbound gRNA, and elution was done with 50ug FLAG peptide (ThermoFisher; A36806) for 15mins with shaking at room temperature to obtain dCas13-B2-EGFP-3xFLAG/sgRNA complex.

    Labeling:

    Article Title: Large RNA polymerase II condensates are promoter-centric assemblies associated with early stages of transcription at all expressed genes
    Article Snippet: The following day, labeled DNA was purified using DNA Clean & Concentrator (Zymo) and labeling efficiency was assessed by UV–Vis spectrophotometry (NanoDrop). .. For probe preparation, 200 ng labeled DNA was ethanol-precipitated together with Cot-1 DNA (4 μg; Thermo), salmon sperm DNA (10 μg; Thermo), and glycogen carrier, incubated at −80°C for 1 h, pelleted by centrifugation, washed twice with 70% ethanol, and air-dried protected from light. ..

    Centrifugation:

    Article Title: Large RNA polymerase II condensates are promoter-centric assemblies associated with early stages of transcription at all expressed genes
    Article Snippet: The following day, labeled DNA was purified using DNA Clean & Concentrator (Zymo) and labeling efficiency was assessed by UV–Vis spectrophotometry (NanoDrop). .. For probe preparation, 200 ng labeled DNA was ethanol-precipitated together with Cot-1 DNA (4 μg; Thermo), salmon sperm DNA (10 μg; Thermo), and glycogen carrier, incubated at −80°C for 1 h, pelleted by centrifugation, washed twice with 70% ethanol, and air-dried protected from light. ..



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