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salmon sperm dna  (Thermo Fisher)


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    Structured Review

    Thermo Fisher salmon sperm dna
    Salmon Sperm Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/salmon+sperm+dna/Deoxyribonucleic+acid+sodium+salt%2C+salmon+testes/pm42243101-259-45-48
    Average 95 stars, based on 1 article reviews
    salmon sperm dna - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Co-enrichment of proteins in extracellular vesicles.
    Article Snippet: .. For on-slide PS staining, FITC-conjugated Annexin V (Biolegend, USA) was diluted 1: 50 in Annexin V binding buffer (Biolegend, USA) and incubated for 1 h. For antibody immunostaining, oligoconjugated antibodies were incubated at 5 μg/mL for 2 h diluted in PBS with 3% BSA, sheared salmon sperm DNA (Thermo Fisher, USA) and 0.5% dextran sulfate (Sigma, USA), and additionally with 0.05% Saponin for cytosolic targets. ..

    Binding Assay:

    Article Title: Co-enrichment of proteins in extracellular vesicles.
    Article Snippet: .. For on-slide PS staining, FITC-conjugated Annexin V (Biolegend, USA) was diluted 1: 50 in Annexin V binding buffer (Biolegend, USA) and incubated for 1 h. For antibody immunostaining, oligoconjugated antibodies were incubated at 5 μg/mL for 2 h diluted in PBS with 3% BSA, sheared salmon sperm DNA (Thermo Fisher, USA) and 0.5% dextran sulfate (Sigma, USA), and additionally with 0.05% Saponin for cytosolic targets. ..

    Incubation:

    Article Title: Co-enrichment of proteins in extracellular vesicles.
    Article Snippet: .. For on-slide PS staining, FITC-conjugated Annexin V (Biolegend, USA) was diluted 1: 50 in Annexin V binding buffer (Biolegend, USA) and incubated for 1 h. For antibody immunostaining, oligoconjugated antibodies were incubated at 5 μg/mL for 2 h diluted in PBS with 3% BSA, sheared salmon sperm DNA (Thermo Fisher, USA) and 0.5% dextran sulfate (Sigma, USA), and additionally with 0.05% Saponin for cytosolic targets. ..

    Article Title: An integrated protocol for multiplexed DNA FISH and protein detection in large tissue sections
    Article Snippet: .. After fixation and permeabilization, tissue samples were incubated at 37°C inside a humidity chamber for 1 h in 70ul pre-hybridization buffer containing 50% formamide / 2x SSC / 5x Denhardt’s solution (ThermoFisher) / 50 mM sodium phosphate buffer / 1 mM EDTA (ThermoFisher) / 100 μg/mL salmon sperm DNA (Invitrogen) (pH 7.75 ± 0.25). .. Then the prehybridization buffer was replaced with by the hybridization mix obtained by mixing the primary FISH probes diluted at 0.6 nM per oligonucleotide, at a ratio of 1:9 vol./vol. in a buffer consisting of 55% formamide / 2.2x SSC / 5.5x Denhardt’s solution / 55 mM sodium phosphate buffer / 1.1 mM EDTA / 111 ng/μL salmon sperm DNA / 11% w/w dextran sulfate (Thermo Fisher) (pH 7.75 ± 0.25).

    Article Title: Large RNA polymerase II condensates are promoter-centric assemblies associated with early stages of transcription at all expressed genes
    Article Snippet: The following day, labeled DNA was purified using DNA Clean & Concentrator (Zymo) and labeling efficiency was assessed by UV–Vis spectrophotometry (NanoDrop). .. For probe preparation, 200 ng labeled DNA was ethanol-precipitated together with Cot-1 DNA (4 μg; Thermo), salmon sperm DNA (10 μg; Thermo), and glycogen carrier, incubated at −80°C for 1 h, pelleted by centrifugation, washed twice with 70% ethanol, and air-dried protected from light. ..

    Immunostaining:

    Article Title: Co-enrichment of proteins in extracellular vesicles.
    Article Snippet: .. For on-slide PS staining, FITC-conjugated Annexin V (Biolegend, USA) was diluted 1: 50 in Annexin V binding buffer (Biolegend, USA) and incubated for 1 h. For antibody immunostaining, oligoconjugated antibodies were incubated at 5 μg/mL for 2 h diluted in PBS with 3% BSA, sheared salmon sperm DNA (Thermo Fisher, USA) and 0.5% dextran sulfate (Sigma, USA), and additionally with 0.05% Saponin for cytosolic targets. ..

    Blocking Assay:

    Article Title: Single-Cell and Spatial Methods for Multimodal Functional Glycan Profiling in Tissues
    Article Snippet: The antibody mixture was then filtered through a EDTA-free S2 Buffer pre-wetted 50kDa filter (Sigma Millipore, UFC5050BK) at 12,500 rpm for 8 min at 4°C. .. The slides were then blocked with Blocking Buffer that contains BBDG (5% normal donkey serum, 0.05% NaN 3 in 1× TBS-T wash buffer (Sigma, 935B-09)) supplemented with 50 μg/ml mouse IgG (diluted from 1 mg/ml stock (Sigma, I5381-10mg) in S2), 50 μg/ml rat IgG (diluted from 1 mg/ml stock (Sigma, I4141-10mg) in S2), 500 μg/ml sheared salmon sperm DNA (ThermoFisher, AM9680), 50 nM oligo block (diluted from stock with 500 nM of each oligo in 1× TE pH 8.0 (Invitrogen, AM9849) and 1X Carbo-free Blocking Solution (diluted from 10X (Vector Laboratories, SP-5040-125)). .. Blocking was performed in a humidity chamber on ice during which was photobleached using Happy Lights (Verilux, VT22) for 1 h, with temperature kept below 40°C.

    other:

    Article Title: An integrated protocol for multiplexed DNA FISH and protein detection in large tissue sections
    Article Snippet: Next, to prepare the samples for hybridization, both cells and tissue samples were rinsed once then incubated overnight in a buffer containing 50% formamide (ThermoFisher) / 2x SSC / 50 mM sodium phosphate buffer, at room temperature in the dark.

    Lysis:

    Article Title: Reprogramming mRNA localization by targeted RNA-protein interference
    Article Snippet: For purification of dCas13-B2-EGFP-3xFLAG/sgRNA complex, initially, dCas13-B2-EGFP-3xFLAG was immunoprecipitated with anti-FLAG magnetic agarose beads (ThermoFisher; A36798), as described earlier, from cell lysates of HEK 293LT that were transiently expressing the protein. .. The bound RNA-λN-GST protein complex was washed with lysis buffer (50mM Tris-Cl pH 7.5, 0.5% Triton X-100, 100mM NaCl, 2.5mM MgCl 2 ) and blocked with lysis buffer containing E. coli tRNA, BSA (Sigma; 10711454001), salmon sperm DNA (Invitrogen; 15632011) and glycogen (Invitrogen; AM9510) for 30–60 min at 4°C. .. Subsequently, beads were washed 5 times with lysis buffer to remove unbound gRNA, and elution was done with 50ug FLAG peptide (ThermoFisher; A36806) for 15mins with shaking at room temperature to obtain dCas13-B2-EGFP-3xFLAG/sgRNA complex.

    Labeling:

    Article Title: Large RNA polymerase II condensates are promoter-centric assemblies associated with early stages of transcription at all expressed genes
    Article Snippet: The following day, labeled DNA was purified using DNA Clean & Concentrator (Zymo) and labeling efficiency was assessed by UV–Vis spectrophotometry (NanoDrop). .. For probe preparation, 200 ng labeled DNA was ethanol-precipitated together with Cot-1 DNA (4 μg; Thermo), salmon sperm DNA (10 μg; Thermo), and glycogen carrier, incubated at −80°C for 1 h, pelleted by centrifugation, washed twice with 70% ethanol, and air-dried protected from light. ..

    Centrifugation:

    Article Title: Large RNA polymerase II condensates are promoter-centric assemblies associated with early stages of transcription at all expressed genes
    Article Snippet: The following day, labeled DNA was purified using DNA Clean & Concentrator (Zymo) and labeling efficiency was assessed by UV–Vis spectrophotometry (NanoDrop). .. For probe preparation, 200 ng labeled DNA was ethanol-precipitated together with Cot-1 DNA (4 μg; Thermo), salmon sperm DNA (10 μg; Thermo), and glycogen carrier, incubated at −80°C for 1 h, pelleted by centrifugation, washed twice with 70% ethanol, and air-dried protected from light. ..



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    Thermo Fisher salmon sperm competitor dna
    ( A ) Computational pipeline to identify vrRNA targeting rules. Four possible non-contiguous recognition patterns where vrRNA’s GGY/NN motifs recognizes NN (0 nt skip), xNN (1 nt skip), xxNN (2 nt skip), or xxxNN (3 nt skip) were tested. ( B ) NN match frequency distributions for each skip rule. Each alignment to the E. coli pan-genome is scored by the percentage of matching vrRNA NN positions (rightward shift indicates higher match quality). (C) VIPR targeting mechanism. Every NN position of vrRNA base-pairs with the target using the 1 nt skip rule. The orange base corresponds to the “skip base”. (D) In vitro competitive binding assay with SUSP1 VIPR (Vipr-vrRNA ribonucleoprotein) using different skip rule substrates (fluorescein-labeled; FAM) and salmon sperm <t>competitor</t> <t>DNA.</t> The competitor to substrate ratio is 0, 1, 2, and 10 fold excess by weight. (E) VIPR mediated green fluorescent protein (GFP) repression assay. Empt vector (EV) was used as negative control. (F) GFP repression assay results with reprogrammed vrRNAs.
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    Image Search Results


    ( A ) Computational pipeline to identify vrRNA targeting rules. Four possible non-contiguous recognition patterns where vrRNA’s GGY/NN motifs recognizes NN (0 nt skip), xNN (1 nt skip), xxNN (2 nt skip), or xxxNN (3 nt skip) were tested. ( B ) NN match frequency distributions for each skip rule. Each alignment to the E. coli pan-genome is scored by the percentage of matching vrRNA NN positions (rightward shift indicates higher match quality). (C) VIPR targeting mechanism. Every NN position of vrRNA base-pairs with the target using the 1 nt skip rule. The orange base corresponds to the “skip base”. (D) In vitro competitive binding assay with SUSP1 VIPR (Vipr-vrRNA ribonucleoprotein) using different skip rule substrates (fluorescein-labeled; FAM) and salmon sperm competitor DNA. The competitor to substrate ratio is 0, 1, 2, and 10 fold excess by weight. (E) VIPR mediated green fluorescent protein (GFP) repression assay. Empt vector (EV) was used as negative control. (F) GFP repression assay results with reprogrammed vrRNAs.

    Journal: bioRxiv

    Article Title: A Noncontiguous Code for RNA-Guided DNA Recognition Preceded CRISPR

    doi: 10.64898/2026.04.26.720920

    Figure Lengend Snippet: ( A ) Computational pipeline to identify vrRNA targeting rules. Four possible non-contiguous recognition patterns where vrRNA’s GGY/NN motifs recognizes NN (0 nt skip), xNN (1 nt skip), xxNN (2 nt skip), or xxxNN (3 nt skip) were tested. ( B ) NN match frequency distributions for each skip rule. Each alignment to the E. coli pan-genome is scored by the percentage of matching vrRNA NN positions (rightward shift indicates higher match quality). (C) VIPR targeting mechanism. Every NN position of vrRNA base-pairs with the target using the 1 nt skip rule. The orange base corresponds to the “skip base”. (D) In vitro competitive binding assay with SUSP1 VIPR (Vipr-vrRNA ribonucleoprotein) using different skip rule substrates (fluorescein-labeled; FAM) and salmon sperm competitor DNA. The competitor to substrate ratio is 0, 1, 2, and 10 fold excess by weight. (E) VIPR mediated green fluorescent protein (GFP) repression assay. Empt vector (EV) was used as negative control. (F) GFP repression assay results with reprogrammed vrRNAs.

    Article Snippet: 1 μΜ of purified SUSP1 VIPR RNP was incubated with 1 μΜ of FAM-labeled target dsDNA and purified salmon sperm competitor DNA (10mg/mL, Invitrogen) in 50mM HEPES (pH 6.8), 100mM KCl, 5mM MgCL 2 , 0.1% glycerol (v/v), and 1mM TCEP.

    Techniques: In Vitro, Competitive Binding Assay, Labeling, Plasmid Preparation, Negative Control

    Diagrams of the different skip rule patterns tested (top). Fluorescein (FAM) channel imaging of 12% native PAGE gel depicting SUSP1 VIPR RNP binding to different skip rule patterned DNA target in a competitive binding assay. Salmon sperm DNA was supplied as a competitor substrate at a ratio of 0, 1, 2, and 10 fold excess by weight compared to the FAM labeled substrate.

    Journal: bioRxiv

    Article Title: A Noncontiguous Code for RNA-Guided DNA Recognition Preceded CRISPR

    doi: 10.64898/2026.04.26.720920

    Figure Lengend Snippet: Diagrams of the different skip rule patterns tested (top). Fluorescein (FAM) channel imaging of 12% native PAGE gel depicting SUSP1 VIPR RNP binding to different skip rule patterned DNA target in a competitive binding assay. Salmon sperm DNA was supplied as a competitor substrate at a ratio of 0, 1, 2, and 10 fold excess by weight compared to the FAM labeled substrate.

    Article Snippet: 1 μΜ of purified SUSP1 VIPR RNP was incubated with 1 μΜ of FAM-labeled target dsDNA and purified salmon sperm competitor DNA (10mg/mL, Invitrogen) in 50mM HEPES (pH 6.8), 100mM KCl, 5mM MgCL 2 , 0.1% glycerol (v/v), and 1mM TCEP.

    Techniques: Imaging, Clear Native PAGE, Binding Assay, Competitive Binding Assay, Labeling